Ocular Irritation and Corrosion
An in vitro screen for Ocular Toxicity, Irritation, and Custom Evaluations using a reconstructed Human Corneal Epithelium (HCE) Model
CeeTox can help you meet Amendment 7 and REACH directives and reduce the need for in vivo testing.
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CeeTox evaluates and characterizes your test articles for their potential to cause toxicity or irritation to the eye using an in vitro 3-D human corneal epithelium (HCE) model, currently being tested in a pre-validation study by ECVAM. Peer-reviewed validation studies show greater than 80% concordance of in vitro results to the Draize eye irritation test. The HCE model ultra-structurally resembles the corneal mucosa of the human eye in both tissue morphology and thickness, and it secretes the same mucins.
Measurement of ocular toxicity begins with an assessment of solubility and preparation of dosing solutions, followed by range-finding to determine exposures and concentrations if required for the sampe, however liquids or solids can be tested in final form. Evaluation is made at multiple timepoints dictated by the suspected measure of irritancy. The magnitude of viability is quantified by using MTT or other metabolically converted vital dyes. The standard screen can be customized with optional assays